antibody

Anti-Phospho-JNK1/2/3(T183+T183+T221) Antibody [ST500]

ET1609-42TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

ST500

human, mouse, rat

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-Phospho-JNK1/2/3(T183+T183+T221) Antibody [ST500]

Human,Mouse,Rat

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr

Non-conjugated

Synthetic phospho-peptide corresponding to residues surrounding Thr183 + Thr183 + Thr221 of Human JNK1 / 2 / 3 aa 161-204 / 427.

SwissProt: P45983 Human;SwissProt: P45984 Human;SwissProt: P53779 Human;SwissProt: Q61831 Mouse;SwissProt: Q91Y86 Mouse;SwissProt: Q9WTU6 Mouse;SwissProt: P49185 Rat;SwissProt: P49186 Rat;SwissProt: P49187 Rat

Rabbit

ST500

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

NIH/3T3 cell lysate treated with Anisomycin, Hela cell lysate treated with Anisomycin, A431 cell lysate treated with UV40, 293 cell lysate treated with UV40, Hela cell lysate treated with UV40, Hela, NIH/3T3, HUVEC, human colon tissue, human endometrium tissue, mouse heart tissue.

Predicted band size: 48/53 kDa

Cytoplasm, Membrane, Mitochondrion, Nucleus.

1 mg/mL.

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:400 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. ;IHC-Fr:1:100

ET1609-42_2.jpg

Western blot analysis of Phospho-JNK1/2/3(T183+T183+T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (ET1609-42) at 1/1,000 dilution. Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with Anisomycin Lane 3: A431 cell lysate, untreated Lane 4: A431 cell lysate, treated with UV40 Lane 5: 293 cell lysate, untreated Lane 6: 293 cell lysate, treated with UV40 Lane 7: Hela cell lysate, untreated Lane 8: Hela cell lysate, treated with UV40 Lysates/proteins at 10 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

ET1609-42_3.jpg

ICC staining of Phospho-JNK1/2/3(T183+T183+T221) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1609-42_4.jpg

ICC staining of Phospho-JNK1/2/3(T183+T183+T221) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1609-42_5.jpg

ICC staining of Phospho-JNK1/2/3(T183+T183+T221) in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1609-42_6.jpg

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (ET1609-42) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1609-42_7.jpg

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (ET1609-42) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1609-42_8.jpg

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (ET1609-42) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1609-42_9.jpg

Flow cytometric analysis of Phospho-JNK1/2/3(T183+T183+T221) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-42, 1/100) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

ET1609-42_10.jpg

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-JNK1/2/3(T183+T183+T221) with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (ET1609-42). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-42, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

ET1609-42_11.jpg

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-JNK1/2/3(T183+T183+T221) with Rabbit anti-Phospho-JNK1/2/3(T183+T183+T221) antibody (ET1609-42). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-42, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.