Phospho-JNK1/2/3 (T183 + T183 + T221) | HUABIO ET1609-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-JNK1/2/3 (T183 + T183 + T221)

catalog :

ET1609-42

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST500

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/1,000 dilution. Lane 1: Hela cell lysate, untreated (10 µg/Lane) Lane 2: Hela cell lysate, treated with Anisomycin (10 µg/Lane) Lane 3: A431 cell lysate, untreated (10 µg/Lane) Lane 4: A431 cell lysate, treated with UV40 (10 µg/Lane) Lane 5: 293 cell lysate, untreated (10 µg/Lane) Lane 6: 293 cell lysate, treated with UV40 (10 µg/Lane) Lane 7: Hela cell lysate, untreated (10 µg/Lane) Lane 8: Hela cell lysate, treated with UV40 (10 µg/Lane) Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/2,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25µg/mL Anisomycin for 30 minutes cell lysate. Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 2 minutes 6 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) on different lysates with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48/53 kDa Observed band size: 48/53 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1609-42

Target name :

Phospho-JNK1/2/3 (T183 + T183 + T221)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr183 of Human JNK1 aa 161-204 / 427.

Uniprot id :

P45983>SwissProt: P45983 Human;SwissProt: P45984 Human;SwissProt: P53779 Human;SwissProt: Q61831 Mouse;SwissProt: Q91Y86 Mouse;SwissProt: Q9WTU6 Mouse;SwissProt: P49185 Rat;SwissProt: P49186 Rat;SwissProt: P49187 Rat

Host :

Rabbit

Clone number :

ST500

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate treated with UV40, 293 cell lysate treated with UV40, Hela cell lysate treated with UV40, HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate, NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, C6 treated with 25ug/mL Anisomycin for 30 minutes cell lysate, Hela, NIH/3T3, HUVEC, human colon tissue, human endometrium tissue, mouse heart tissue, rat liver tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 48/53 kDa

Subcellular location :

Cytoplasm, Membrane, Mitochondrion, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:100-1:200 ;IF-Tissue: 1:200-1:500 ;IHC-P: 1:400-1:1,000 ;FC: 1:500-1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-Fr:1:100-1:500

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_4.jpg

Pic legend4 :

ICC staining of Phospho-JNK1/2/3 (T183 + T183 + T221) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_5.jpg

Pic legend5 :

ICC staining of Phospho-JNK1/2/3 (T183 + T183 + T221) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_6.jpg

Pic legend6 :

ICC staining of Phospho-JNK1/2/3 (T183 + T183 + T221) in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-42) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_12.jpg

Pic legend12 :

Flow cytometric analysis of Phospho-JNK1/2/3 (T183 + T183 + T221) was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-42, 1/100) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_13.jpg

Pic legend13 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-JNK1/2/3 (T183 + T183 + T221) with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-42, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/ET1609-42_14.jpg

Pic legend14 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-JNK1/2/3 (T183 + T183 + T221) with Rabbit anti-Phospho-JNK1/2/3 (T183 + T183 + T221) antibody (ET1609-42). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-42, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

more info or order :

HUABIO product webpage