product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-Phospho-Smad3(S423/S425) Antibody [ST0493]
catalog :
ET1609-41TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
ST0493
reactivity :
human, mouse
application :
western blot, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
ET1609-41TR
Target name :
Anti-Phospho-Smad3(S423/S425) Antibody [ST0493]
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell,IF-Tissue,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.
Uniprot id :
SwissProt: P84022 Human;SwissProt: Q8BUN5 Mouse
Host :
Rabbit
Clone number :
ST0493
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, K562 cell lysates, A549 cell lysates, A431, human large intestine tissue, mouse skin tissue, mouse kidney tissue.
Molecular wt :
Predicted band size: 48 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:50-1:500
Pic img4 :
ET1609-41_2.jpg
Pic img5 :
Western blot analysis of Phospho-Smad3(S423/S425) on K562 cell lysates.
Lane 1: K562 cells, whole cell lysate, 10ug/lane
Lane 2: K562 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane
All lanes :
Anti-Phospho-Smad3(S423/S425) antibody (ET1609-41) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.
Predicted band size: 48 kDa
Observed band size: 60 kDa
Blocking and diluting buffer: 5% BSA.
Exposure time: 3 minutes 43 seconds
Pic legend5 :
ET1609-41_3.jpg
Pic legend6 :
Western blot analysis of Phospho-Smad3(S423/S425) on A549 cell lysates.
Lane 1 : A549 cells, whole cell lysate, 10 μg /lane.
Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 μg/lane.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Phospho-Smad3(S423/S425)(ET1609-41, 1/500) , Anti-Smad3 antibody ( ET1607-41, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 48 kDa
Observed band size: 60 kDa
Exposure time: 3 seconds
Pic img7 :
ET1609-41_4.jpg
Pic img8 :
ICC staining Phospho-Smad3(S423/S425) (1/50) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic legend8 :
ET1609-41_5.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Phospho-Smad3(S423/S425) antibody (ET1609-41) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
ET1609-41_6.jpg
Pic img11 :
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Phospho-Smad3(S423/S425) antibody (ET1609-41) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-41) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend11 :
ET1609-41_7.jpg
Pic legend12 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Smad3(S423/S425) antibody (ET1609-41) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-41) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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