ATP citrate lyase | HUABIO ET1609-37 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATP citrate lyase

catalog :

ET1609-37

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST51-07

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ATP citrate lyase with anti-ATP citrate lyase antibody [ST51-07] (ET1609-37) at 1/1,000 dilution. Lane 1: Wild-type A549 whole cell lysate (20 µg). Lane 2: ATP citrate lyase knockout A549 whole cell lysate (20 µg). ET1609-37 was shown to specifically react with ATP citrate lyase in wild-type A549 cells. No band was observed when ATP citrate lyase knockout sample was tested. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-37, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ATP citrate lyase on different lysates with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-37) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of ATP citrate lyase on different lysates with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1609-37

Target name :

ATP citrate lyase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human ATP citrate lyase.

Uniprot id :

P53396>SwissProt: P53396 Human;SwissProt: Q91V92 Mouse;SwissProt: P16638 Rat

Host :

Rabbit

Clone number :

ST51-07

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, C6 cell lysate, HeLa, NIH/3T3, human kidney tissue, mouse kidney tissue, rat kidney tissue, human thyroid tissue.

Molecular wt :

Predicted band size: 121 kDa

Subcellular location :

Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:1,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling ATP citrate lyase with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_5.jpg

Pic legend5 :

Immunocytochemistry analysis of NIH/3T3 cells labeling ATP citrate lyase with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ATP citrate lyase antibody (ET1609-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ATP citrate lyase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_10.jpg

Pic legend10 :

Flow cytometric analysis of HeLa cells labeling ATP citrate lyase. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-37, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_11.jpg

Pic legend11 :

Flow cytometric analysis of NIH/3T3 cells labeling ATP citrate lyase. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-37, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1609-37_12.jpg

Pic legend12 :

ATP citrate lyase was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with ET1609-37 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1609-37 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: ET1609-37 IP in NIH/3T3 cell lysate Lane 3: Rabbit IgG instead of ET1609-37 in NIH/3T3 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801

more info or order :

HUABIO product webpage