Phospho-GATA3 (S308) | HUABIO ET1609-17 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-GATA3 (S308)

catalog :

ET1609-17

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST44-09

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-GATA3 (S308) on different lysates with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat cell lysate, treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-17) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-GATA3 (S308) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human skin tissue lysate Lane 2: Jurkat cell lysate

image 3 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1609-17

Target name :

Phospho-GATA3 (S308)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser308 of Human GATA3 aa 281-$330 / 443.

Uniprot id :

P23771>SwissProt: P23771 Human;SwissProt: P23772 Mouse;Entrez Gene: 85471 Rat

Host :

Rabbit

Clone number :

ST44-09

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, human skin tissue lysate, human stomach tissue, mouse stomach tissue, rat stomach tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration. ;IF-Tissue: 1:50

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1609-17_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1609-17_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1609-17_6.jpg

Pic legend6 :

Application: IF-Tissue Species: Mouse Site: stomach Sample: Paraffin-embedded section Antibody concentration: 1/50

more info or order :

HUABIO product webpage