product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Phospho-p53 (S376)
catalog :
ET1609-14
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
ST0440
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Phospho-p53 (S376) on different lysates with Rabbit anti-Phospho-p53 (S376) antibody (ET1609-14) at 1/1,000 dilution.
Lane 1: HEK-293 whole cell lysate
Lane 2: HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-14) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Phospho-p53 (S376) on different lysates with Rabbit anti-Phospho-p53 (S376) antibody (ET1609-14) at 1/1,000 dilution.
Lane 1: RAW264.7 whole cell lysate
Lane 2: RAW264.7 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ET1609-14
Target name :
Phospho-p53 (S376)
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human p53 aa 344-393 / 393.
Uniprot id :
P04637>SwissProt: P04637 Human
Host :
Rabbit
Clone number :
ST0440
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate, RAW264.7 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments