Phospho-p53 (T55) | HUABIO ET1609-13 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-p53 (T55)

catalog :

ET1609-13

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST0439

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-p53 (T55) on different lysates with Rabbit anti-Phospho-p53 (T55) antibody (ET1609-13) at 1/1,000 dilution. Lane 1: HEK-293 whole cell lysate Lane 2: HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-13) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Phospho-p53 (T55) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-13, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1609-13

Target name :

Phospho-p53 (T55)

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr55 of Human p53 aa 31-80 / 393.

Uniprot id :

P04637>SwissProt: P04637 Human

Host :

Rabbit

Clone number :

ST0439

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate, human esophagus tissue.

Molecular wt :

Predicted band size: 53 kDa

Subcellular location :

Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix, Cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage