p16INK4a | HUABIO ET1608-62-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p16INK4a

catalog :

ET1608-62-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU0702

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p16INK4a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-62, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: SiHa cell lysate

image 2 :

Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (ET1608-62) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si p16INK4a cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1608-62-50UL

Target name :

p16INK4a

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human p16INK4a aa 107-156 / 156.

Uniprot id :

P42771>SwissProt: P42771 Human

Host :

Rabbit

Clone number :

SU0702

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293 cell lysate, SiHa cell lysate, HepG2, Hela, PC-3M, human colon carcinoma tissue, human cervical carcinoma tissue, human ovary carcinoma tissue.

Molecular wt :

Predicted band size: 16 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage