antibody

Anti-mTOR Antibody [SU30-00]

ET1608-5TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SU30-00

human, mouse, rat

western blot, immunoprecipitation, immunohistochemistry - paraffin section

Anti-mTOR Antibody [SU30-00]

Human,Mouse,Rat

WB,IHC-P,IP,IF-Cell

Non-conjugated

Synthetic peptide within human mTOR aa 2400-2440.

SwissProt: P42345 Human;SwissProt: Q9JLN9 Mouse;SwissProt: P42346 Rat

Rabbit

SU30-00

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat heart tissue lysate, SiHa cell lysate, human breast carcinoma tissue, HeLa, NIH/3T3, human kidney tissue, mouse testis tissue, mouse kidney tissue.

Predicted band size: 289 kDa

Endoplasmic reticulum membrane, Microsome membrane, PML body, Golgi apparatus membrane, Cytoplasm, Lysosome, Lysosome membrane, Mitochondrion outer membrane, phagosome.

1 mg/mL.

WB: 1:5,000 ;IHC-P: 1:50-1:200 ;IF-cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.

ET1608-5_2.jpg

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1608-5_3.jpg

Immunocytochemistry analysis of HeLa cells labeling mTOR with Rabbit anti-mTOR antibody (ET1608-5) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mTOR antibody (ET1608-5) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

ET1608-5_4.jpg

Immunocytochemistry analysis of NIH/3T3 cells labeling mTOR with Rabbit anti-mTOR antibody (ET1608-5) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mTOR antibody (ET1608-5) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

ET1608-5_5.jpg

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1608-5_6.jpg

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1608-5_7.jpg

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-mTOR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.