PARP1 | HUABIO ET1608-56 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PARP1

catalog :

ET1608-56

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU03-68

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: C2C12 cell lysate (15 µg/Lane) Lane 5: C6 cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ET1608-56) at 1/2,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113/28 kDa Exposure time: 1 minute 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 3 :

All lanes: Western blot analysis of PARP with anti-PARP1 antibody[SU03-68] (ET1608-56) at 1:500 dilution. Lane 1/2: Wild-type Hela whole cell lysate (10 µg). Lane 3/4: PARP knockdown Hela whole cell lysate (10 µg). ET1608-56 was shown to specifically react with PARP in wild-type Hela cells. Weakened bands were observed when PARP knockdown samples were tested. Wild-type and PARP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1608-56

Target name :

PARP1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human PARP1.

Uniprot id :

P09874>SwissProt: P09874 Human;SwissProt: P11103 Mouse;SwissProt: P27008 Rat

Host :

Rabbit

Clone number :

SU03-68

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, HeLa whole cell lysate, HeLa treated with 1μM staurosporine for 3 hours whole cell lysate, HeLa, human breast cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue.

Molecular wt :

Predicted band size: 113 kDa

Subcellular location :

Nucleus, nucleolus, chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:1,000 ;IHC-P: 1:4,000 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling PARP1 with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution. Cells were fixed in 100% methanol for 5 minutes, blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_9.jpg

Pic legend9 :

Flow cytometric analysis of HeLa cells labeling PARP1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-56, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1608-56_10.jpg

Pic legend10 :

Application: IF-Tissue Species: Human Site: breast cancer Sample: Paraffin-embedded section Antibody concentration: 1/1,000

more info or order :

HUABIO product webpage