CaMKII | HUABIO ET1608-47 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CaMKII

catalog :

ET1608-47

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU03-57

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CaMKII on different lysates with Rabbit anti-CaMKII antibody (ET1608-47) at 1/1,000 dilution. Lane 1: SHG-44 cell lysate Lane 2: PC-12 cell lysate Lane 3: Neuro-2a cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-47) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of PC-12 cells labeling CaMKII with Rabbit anti-CaMKII antibody (ET1608-47) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CaMKII antibody (ET1608-47) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMKII antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1608-47

Target name :

CaMKII

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IHC-Fr,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CaMKⅡ aa 201-250 / 478.

Uniprot id :

Q13554>SwissProt: Q13554 Human;SwissProt: Q13555 Human;SwissProt: Q13557 Human;SwissProt: Q9UQM7 Human;SwissProt: P11798 Mouse;SwissProt: P28652 Mouse;SwissProt: Q6PHZ2 Mouse;SwissProt: Q923T9 Mouse;SwissProt: P08413 Rat;SwissProt: P11275 Rat;SwissProt: P11730 Rat;SwissProt: P15791 Rat

Host :

Rabbit

Clone number :

SU03-57

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SHG-44 cell lysate, PC-12 cell lysate, Neuro-2a cell lysate, PC-12, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cytoplasm, Sarcoplasmic reticulum membrane, Cell membrane, Cell junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IHC-Fr: 1:100 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1608-47_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMKII antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1608-47_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CaMKII antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1608-47_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1608-47_7.jpg

Pic legend7 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling CaMKⅡ with Rabbit anti-CaMKⅡ antibody (ET1608-47). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1608-47, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1608-47_8.jpg

Pic legend8 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling CaMKⅡ with Rabbit anti-CaMKⅡ antibody (ET1608-47). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1608-47, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1608-47_9.jpg

Pic legend9 :

Flow cytometric analysis of PC-12 cells labeling CaMKII. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-47, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage