Phospho-c-Jun (S63) | HUABIO ET1608-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-c-Jun (S63)

catalog :

ET1608-4

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY0297

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-c-Jun (S63) on different lysates with Rabbit anti-Phospho-c-Jun (S63) antibody (ET1608-4) at 1/2,000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 5: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes, then treated with λpp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of Phospho-c-Jun (S63) in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Western blot analysis of Phospho-c-Jun (S63) on different lysates with Rabbit anti-Phospho-c-Jun (S63) antibody (ET1608-4) at 1/500 dilution. Lane 1: A549 whole cell lysate Lane 2: A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-4) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1608-4

Target name :

Phospho-c-Jun (S63)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser63 of Human c-Jun aa 31-80 / 331.

Uniprot id :

P05412>SwissProt: P05412 Human;SwissProt: P05627 Mouse;SwissProt: P17325 Rat

Host :

Rabbit

Clone number :

SY0297

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate, NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate, PC-3M, MCF-7, A549, human breast carcinoma tissue, human tonsil tissue, human colon carcinoma tissue, human endometrial tissue.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:500

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1608-4_4.jpg

Pic legend4 :

ICC staining of Phospho-c-Jun (S63) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1608-4_5.jpg

Pic legend5 :

ICC staining of Phospho-c-Jun (S63) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1608-4_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun (S63) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1608-4_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun (S63) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1608-4_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Phospho-c-Jun (S63) antibody (ET1608-4) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-4) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1608-4_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human endometrial tissue with Rabbit anti-Phospho-c-Jun (S63) antibody (ET1608-4) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-4) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage