5T4 | HUABIO ET1608-37 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

5T4

catalog :

ET1608-37

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU34-05

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: Human lung tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: Lane 1: 25 seconds; Lane 2: 2 minutes 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of 5T4 on SK-Br-3 cell lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-5T4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1608-37

Target name :

5T4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human 5T4 aa 381-420 / 420.

Uniprot id :

Q13641>SwissProt: Q13641 Human;SwissProt: Q9Z0L0 Mouse;SwissProt: Q5PQV5 Rat

Host :

Rabbit

Clone number :

SU34-05

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, Human lung tissue lysate, SK-Br-3 cell lysates, human placenta tissue.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1608-37_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage