LGR5 | HUABIO ET1608-18 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LGR5

catalog :

ET1608-18

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU32-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LGR5 on different lysates with Rabbit anti-LGR5 antibody (ET1608-18) at 1/5,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-18) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of LGR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spinal cord tissue lysate Lane 2: Human skeletal muscle tissue lysate Predicted band size: 100 kDa Observed band size: 100 kDa

image 3 :

Western blot analysis of LGR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat skeletal muscle tissue lysate Lane 2: Rat kidney tissue lysate Lane 3: Rat spinal cord tissue lysate Predicted band size: 100 kDa Observed band size: 100 kDa

product information

SKU :

ET1608-18

Target name :

LGR5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human LGR5 aa 816-865 / 907.

Uniprot id :

O75473>SwissProt: O75473 Human;SwissProt: Q9Z1P4 Mouse;Unigene:214063 Rat

Host :

Rabbit

Clone number :

SU32-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse spinal cord tissue lysate, human skeletal muscle tissue lysate, rat kidney tissue lysate, rat spinal cord tissue lysate, SH-SY5Y.

Molecular wt :

Predicted band size: 100 kDa

Subcellular location :

Cell membrane, Golgi apparatus, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: Use at an assay dependent concentration.

more info or order :

HUABIO product webpage