Anti-Cleaved PARP Antibody [SU0314] | HUABIO ET1608-10TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Cleaved PARP Antibody [SU0314]

catalog :

ET1608-10TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU0314

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

product information

SKU :

ET1608-10TR

Target name :

Anti-Cleaved PARP Antibody [SU0314]

Species reactivity :

Human

Applications :

WB,IF-Cell,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PARP1 aa 200-249 / 1,014.

Uniprot id :

SwissProt: P09874 Human

Host :

Rabbit

Clone number :

SU0314

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, A549 cell lysate, Hela, Daudi cell lysates.

Molecular wt :

Predicted band size: 89 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;FC: 1:50-1:100 ;IF-Cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Pic img4 :

ET1608-10_2.jpg

Pic img5 :

Western blot analysis of Cleaved PARP on different lysates with Rabbit anti-Cleaved PARP antibody (ET1608-10) at 1/500 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si Cleaved PARP cell lysate (10 µg/Lane) Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1608-10 was shown to specifically react with Cleaved PARP in Hela-si NT cells. No band was observed when Hela-si Cleaved PARP sample was tested. Hela-si NT and Hela-si Cleaved PARP samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-10, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Pic legend5 :

ET1608-10_3.jpg

Pic legend6 :

Western blot analysis of Cleaved PARP on Daudi cell lysates with Rabbit anti-Cleaved PARP antibody (ET1608-10) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Pic img7 :

ET1608-10_4.jpg

Pic img8 :

Flow cytometric analysis of Cleaved PARP was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-10, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage