product summary
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company name :
HUABIO
product type :
antibody
product name :
Rb
catalog :
ET1607-9-50UL
quantity :
50μl
price :
205.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SY63-03
reactivity :
human, mouse
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Rb on mouse lung tissue lysates with Rabbit anti-Rb antibody (ET1607-9) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
ICC staining of Rb in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
image 3 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Rb antibody (ET1607-9) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-9) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1607-9-50UL
Target name :
Rb
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse Rb aa 682-921 / 921.
Uniprot id :
P06400>SwissProt: P06400 Human;SwissProt: P13405 Mouse
Host :
Rabbit
Clone number :
SY63-03
Isotype :
IgG
Size :
50μl
List Price :
205.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse lung tissue lysates, SW480, human spleen tissue, human breast carcinoma tissue, human colon tissue, human colon carcinoma tissue.
Molecular wt :
Predicted band size: 106 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:50-1:2,000
;IP: Use at an assay dependent concentration.
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1607-9_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Rb antibody (ET1607-9) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-9) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1607-9_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Rb antibody (ET1607-9) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-9) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1607-9_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Rb antibody (ET1607-9) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-9) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1607-9_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Rb antibody (ET1607-9) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1607-9_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Rb antibody (ET1607-9) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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