VPS34 | HUABIO ET1607-74 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

VPS34

catalog :

ET1607-74

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY0286

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-VPS34 antibody (ET1607-74) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VPS34 antibody (ET1607-74) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VPS34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1607-74

Target name :

VPS34

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human VPS34 aa 830-870.

Uniprot id :

Q8NEB9>SwissProt: Q8NEB9 Human;SwissProt: Q6PF93 Mouse;SwissProt: O88763 Rat

Host :

Rabbit

Clone number :

SY0286

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human stomach cancer tissue, mouse brain tissue, rat brain tissue, A549.

Molecular wt :

Predicted band size: 102 kDa

Subcellular location :

Midbody, Late endosome, Cytoplasmic vesicle.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:50-1:200 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1607-74_4.jpg

Pic legend4 :

Flow cytometric analysis of A549 cells labeling VPS34. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1607-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage