product summary
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company name :
HUABIO
product type :
antibody
product name :
Topoisomerase II alpha
catalog :
ET1607-59
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SY27-00
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Topoisomerase II alpha on different lysates with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
Lane 1: Rat testis tissue lysate (40 µg/Lane)
Lane 2: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Predicted band size: 174 kDa
Observed band size: 174 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 5: 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-59) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Topoisomerase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1607-59
Target name :
Topoisomerase II alpha
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Topoisomerase Ⅱ alphaaa 1,482-1,531 / 1,531.
Uniprot id :
P11388>SwissProt: P11388 Human;SwissProt: Q01320 Mouse;SwissProt: P41516 Rat
Host :
Rabbit
Clone number :
SY27-00
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Rat testis tissue lysate, Mouse testis tissue lysate, NIH/3T3 cell lysate, HT-29 cell lysate, Jurkat cell lysate, human tonsil tissue, human testis tissue, mouse testis tissue, rat testis tissue.
Molecular wt :
Predicted band size: 174 kDa
Subcellular location :
Nucleoplasm, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IHC-P: 1:200-1:1,000
;IP: Use at an assay dependent concentration.
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1607-59_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1607-59_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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