JAK2 | HUABIO ET1607-35 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

JAK2

catalog :

ET1607-35

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY0245

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si JAK2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1607-35

Target name :

JAK2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human JAK2.

Uniprot id :

O60674>SwissProt: O60674 Human;SwissProt: Q62120 Mouse;SwissProt: Q62689 Rat

Host :

Rabbit

Clone number :

SY0245

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

TF-1 cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Jurkat cell lysate, MEF cell lysate, A549, MCF-7, NIH/3T3.

Molecular wt :

Predicted band size: 131 kDa

Subcellular location :

Endomembrane system, Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100-1:500

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1607-35_4.jpg

Pic legend4 :

ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1607-35_5.jpg

Pic legend5 :

ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

more info or order :

HUABIO product webpage