Synaptophysin | HUABIO ET1606-56 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Synaptophysin

catalog :

ET1606-56

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SJ26-85

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

image 2 :

Application: IF-tissue Species: Mouse Site: Pancreas Sample: Paraffin-embedded section Antibody concentration: 1/500

image 3 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500

product information

SKU :

ET1606-56

Target name :

Synaptophysin

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Synaptophysin aa 224 – 313 (Cytoplasmic).

Uniprot id :

P08247>SwissProt: P08247 Human;SwissProt: Q62277 Mouse;SwissProt: P07825 Rat

Host :

Rabbit

Clone number :

SJ26-85

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, atypical carcinoid tissue, human medullary thyroid carcinoma tissue, human pancreas tissue, human small intestine tissue, mouse cerebellum tissue, mouse pancreas tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasmic, Cell junction, synapse, synaptosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:10,000 ;IHC-P: 1:2,000 ;IHC-Fr: 1:1,000 ;IF-Tissue: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_4.jpg

Pic legend4 :

Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: NIH/3T3 cell lysate (negative) Lane 4: PC-12 cell lysate Lane 5: Human brain tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34/40 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-56) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded atypical carcinoid tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1606-56_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage