Integrin beta 3 | HUABIO ET1606-49 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Integrin beta 3

catalog :

ET1606-49

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SJ19-09

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Integrin beta 3 on U-87 MG cell lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/500 dilution. Lane 1: U87-MG-si NT cell lysate Lane 2: U87-MG-si Integrin beta 3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 87,100 kDa Exposure time: 1 minutes 10 seconds; 4-20% SDS-PAGE gel. ET1606-49 was shown to specifically react with Integrin beta 3 in U87-MG-si NT cells. Weakened band was observed when U87-MG-si Integrin beta 3 sample was tested. U87-MG-si NT and U87-MG-si Integrin beta 3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-49, 1/500) and Loading control antibody (Rabbit anti-Hsp90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-49) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1606-49

Target name :

Integrin beta 3

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Integrin beta 3 aa 729-788 / 788.

Uniprot id :

P05106>SwissProt: P05106 Human;SwissProt: O54890 Mouse;Entrez Gene: 29302 Rat

Host :

Rabbit

Clone number :

SJ19-09

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-87 MG cell lysates, HepG2, human spleen tissue, mouse testis tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse colon tissue, U87-MG-si-NT cell lysate.

Molecular wt :

Predicted band size: 87 kDa

Subcellular location :

Cell membrane, Cell projection, Cell junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:400-1:1,000 ;IF-Tissue: 1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1606-49_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage