Phospho-EGFR (Y1092) | HUABIO ET1606-44 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-EGFR (Y1092)

catalog :

ET1606-44

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SJ0194

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-EGFR (Y1092) on different lysates with Rabbit anti-Phospho-EGFR (Y1092) antibody (ET1606-44) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Phospho-EGFR (Y1092) antibody (ET1606-44) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-44) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1606-44

Target name :

Phospho-EGFR (Y1092)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr1092 of Human EGFR aa 1,071-1,120 / 1,210.

Uniprot id :

P00533>SwissProt: P00533 Human;SwissProt: Q01279 Mouse

Host :

Rabbit

Clone number :

SJ0194

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 treated with 100ng/mL EGF for 30 minutes cell lysate, human lung cancer tissue.

Molecular wt :

Predicted band size: 134 kDa

Subcellular location :

Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Tissue: 1:200 ;IHC-P: 1:3,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage