MMP-2 | HUABIO ET1606-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MMP-2

catalog :

ET1606-4

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SI15-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MMP-2 on different lysates with Rabbit anti-MMP-2 antibody (ET1606-4) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: L6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MMP-2 antibody (ET1606-4) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-4) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

MMP-2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with ET1606-4 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1606-4 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input) Lane 2: ET1606-4 IP in U-87 MG cell lysate Lane 3: Rabbit IgG instead of ET1606-4 in U-87 MG cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801

product information

SKU :

ET1606-4

Target name :

MMP-2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human MMP2 aa 101-150 / 660.

Uniprot id :

P08253>SwissProt: P08253 Human;SwissProt: P33434 Mouse;SwissProt: P33436 Rat

Host :

Rabbit

Clone number :

SI15-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-87 MG cell lysate, L6 cell lysate, mouse liver tissue.

Molecular wt :

Predicted band size: 74 kDa

Subcellular location :

Secreted, extracellular space, extracellular matrixm Membrane, Nucleus; Cytoplasm, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:2,000 ;IP: 1-2μg/sample ;IF-Tissue: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1606-4_4.jpg

Pic legend4 :

Application: IF-Tissue Species: Mouse Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/1,000

more info or order :

HUABIO product webpage