product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-Lamin B1 Antibody [SI17-07]
catalog :
ET1606-27TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SI17-07
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
ET1606-27TR
Target name :
Anti-Lamin B1 Antibody [SI17-07]
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Tissue,IHC-P,CUT&Tag-seq
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Lamin B1 aa 511-560 / 586.
Uniprot id :
SwissProt: P20700 Human;SwissProt: P14733 Mouse;SwissProt: P70615 Rat
Host :
Rabbit
Clone number :
SI17-07
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, human liver tissue, human colon carcinoma tissue, human breast carcinoma tissue, mouse colon tissue, mouse brain tissue, PC-12 cell lysate, human lymph nodes tissue, mouse large intestine tissue, HepG2.
Molecular wt :
Predicted band size: 66 kDa
Subcellular location :
Nucleus inner membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IF-Tissue: 1:50-1:200
;IHC-P: 1:500-1:1,000
Pic img4 :
ET1606-27_2.jpg
Pic img5 :
Western blot analysis of Lamin B1 on PC-12 cell lysates with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-27) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Pic legend5 :
ET1606-27_3.jpg
Pic legend6 :
All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody [SI17-06] (ET1606-27) at 1:1,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
Lane 3/4: Lamin B1 knockout Hela whole cell lysate (20 µg).
ET1606-27 was shown to specifically react with Lamin B1 in wild-type Hela cells. No band was observed when Lamin B1 knockout samples were tested. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-27, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, ET1705-94, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Pic img7 :
ET1606-27_4.jpg
Pic img8 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend8 :
ET1606-27_5.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
ET1606-27_6.jpg
Pic img11 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend11 :
ET1606-27_7.jpg
Pic legend12 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img13 :
ET1606-27_8.jpg
Pic img14 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Lamin B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend14 :
ET1606-27_9.jpg
Pic legend15 :
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img16 :
ET1606-27_10.jpg
Pic img17 :
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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