Phospho-p53 (S392) | HUABIO ET1606-24 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-p53 (S392)

catalog :

ET1606-24

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SI17-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-p53 (S392) on different cell lysates with Rabbit anti-Phospho-p53 (S392) antibody (ET1606-24) at 1:1,000 dilution. Lane 1: 293 cell lysate Lane 2: F9 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 30 Seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA for 1 hour at room temperature. The primary antibody (ET1606-24) at 1:1,000 dilution was used in PBS at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-p53(S392) on 293 cell lysates. Lane 1: 293 cells, whole cell lysate, 10ug/lane Lane 2: 293 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-p53(S392) antibody (ET1606-24) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 53 kDa Observed band size: 53 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 10 seconds

image 3 :

Western blot analysis of Phospho-p53(S392) on A549 cell lysates. Lane 1: A549 cells, whole cell lysate, 10ug/lane Lane 2/3: A549 cells treated with 250nM Doxorubicin overnight, whole cell lysates, 10ug/lane Lane 4: A549 cells treated with 250nM Doxorubicin overnight, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-p53(S392) antibody (ET1606-24) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 53 kDa Observed band size: 53 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 5 minutes

product information

SKU :

ET1606-24

Target name :

Phospho-p53 (S392)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser392 of Human p53 aa 344-393 / 393.

Uniprot id :

P04637>SwissProt: P04637 Human;SwissProt: P02340 Mouse;SwissProt: P10361 Rat

Host :

Rabbit

Clone number :

SI17-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293 cell lysate, F9 cell lysate, A431 cell lysate, human stomach carcinoma tissue, mouse prostate tissue, A549 cells.

Molecular wt :

Predicted band size: 53 kDa

Subcellular location :

Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1606-24_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Phospho-p53 (S392) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1606-24_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Phospho-p53 (S392) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1606-24_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue untreaed and treated with λ-PPase with Rabbit anti-Phospho-p53 (S392) antibody (ET1606-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage