ATM | HUABIO ET1606-20 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATM

catalog :

ET1606-20

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SI70-01

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (ET1606-20) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NCCIT cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 24 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-20) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (ET1606-20) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: CRC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 25 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (ET1606-20) at 1/2,000 dilution. Lane 1: A549 WT cell lysate Lane 2: A549 ATM KO cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 10 seconds; ECL: Ori Supersensitive 4-20% SDS-PAGE gel. ET1606-20 was shown to specifically react with ATM in A549 WT cells. No band was observed when A549 ATM KO sample was tested. A549 WT and A549 ATM KO samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-20, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1606-20

Target name :

ATM

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ATM aa 1,951-2,000 / 3,056.

Uniprot id :

Q13315>SwissProt: Q13315 Human;Entrez Gene: 300711 Rat

Host :

Rabbit

Clone number :

SI70-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, NCCIT cell lysate, HEK-293 cell lysate, CRC cell lysate, HeLa, human testis tissue, human tonsil tissue, rat colon tissue, human pancreas tissue.

Molecular wt :

Predicted band size: 351 kDa

Subcellular location :

Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome By Similarity.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:1,000

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1606-20_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling ATM with Rabbit anti-ATM antibody (ET1606-20) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATM antibody (ET1606-20) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1606-20_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATM antibody (ET1606-20) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1606-20_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ATM antibody (ET1606-20) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1606-20_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-ATM antibody (ET1606-20) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-20) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1606-20_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ATM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage