ALDH1A1 | HUABIO ET1605-24 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ALDH1A1

catalog :

ET1605-24

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY11-02

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ALDH1A1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:200 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1605-24

Target name :

ALDH1A1

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human ALDH1A1 aa 300-360.

Uniprot id :

P00352>SwissProt: P00352 Human;SwissProt: P24549 Mouse;SwissProt: P51647 Rat

Host :

Rabbit

Clone number :

SY11-02

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, mouse lung tissue lysate, human liver tissue lysate, human kidney tissue lysate, human liver tissue, human kidney tissue, mouse striatum tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cytoplasm,Cell projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:100-1:200 ;IHC-Fr: 1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1605-24_4.jpg

Pic legend4 :

Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/2,000 dilution. Lane 1: mouse lung tissue lysate Lane 2: human liver tissue lysate Lane 3: human kidney tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1605-24_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage