product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-PMS2 Antibody [SY08-09]
catalog :
ET1605-1TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SY08-09
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
ET1605-1TR
Target name :
Anti-PMS2 Antibody [SY08-09]
Species reactivity :
Human
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human PMS2 aa 1-50 / 862.
Uniprot id :
SwissProt: P54278 Human
Host :
Rabbit
Clone number :
SY08-09
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, U-2 OS cell lysate, human colon carcinoma tissue, human breast carcinoma tissue.
Molecular wt :
Predicted band size: 96 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/ml.
Recommended dilutions :
WB: 1:2,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:50-1:200
;IP: Use at an assay dependent concentration.
;FC: 1:1,000
Pic img4 :
ET1605-1_2.jpg
Pic img5 :
Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (negative) (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Predicted band size: 96 kDa
Observed band size: 120 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Pic legend5 :
ET1605-1_3.jpg
Pic legend6 :
Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HCT 116 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 120 kDa
Exposure time: 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Pic img7 :
ET1605-1_4.jpg
Pic img8 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend8 :
ET1605-1_5.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
ET1605-1_6.jpg
Pic img11 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend11 :
ET1605-1_7.jpg
Pic legend12 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img13 :
ET1605-1_8.jpg
Pic img14 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend14 :
ET1605-1_9.jpg
Pic legend15 :
Flow cytometric analysis of HeLa (positive, red) and HCT 116 (negative, green) cells labeling PMS2.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1605-1, red) at 1/1,000 dilution and competitor's antibody (red) at 1/200 dilution, compared with Rabbit IgG Isotype Control (HeLa black, HCT 116 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!