Olig2 | HUABIO ET1604-29 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Olig2

catalog :

ET1604-29

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP07-02

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

image 3 :

Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NeuN (ET1602-12, red), anti-Iba1 (ET1705-78, green), anti-GFAP (ET1601-23, gray), anti-Olig2 (ET1604-29, cyan), anti-MAP2 (HA500177, magenta) and anti-CD34 (ET1606-11, yellow) on mouse brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in six rounds of staining: in the order of ET1602-12(1/5,000 dilution), ET1705-78 (1/2,000 dilution), ET1601-23 (1/5,000 dilution), ET1604-29 (1/1,000 dilution), HA500177 (1/5,000 dilution) and ET1606-11 (1/2,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

product information

SKU :

ET1604-29

Target name :

Olig2

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,mIHC,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Olig2 aa 238-287 / 323.

Uniprot id :

Q13516>SwissProt: Q13516 Human;SwissProt: Q9EQW6 Mouse;Unigene:22121 Rat

Host :

Rabbit

Clone number :

SP07-02

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue lysate, mouse cerebral cortex tissue, human glioma tissue, human brain tissue, rat brain tissue, mouse brain tissue, mouse hippocampus tissue, E14.5 mouse embryonic brain tissue, mouse glial cells.

Molecular wt :

Predicted band size: 32 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:200 ;mIHC: 1:1,000-1:5,000 ;IF-Cell: 1:200

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_4.jpg

Pic legend4 :

Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-MAP2 (HA500177, Red), anti-Olig2 (ET1604-29, Cyan), anti-GFAP (ET1601-23, Magenta) and anti-Neun (ET1602-12, Yellow) on mouse brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in four rounds of staining: in the order of HA500177 (1/1,000 dilution), ET1604-29 (1/5,000 dilution), ET1601-23 (1/10,000 dilution) and ET1602-12 (1/10,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_5.jpg

Pic legend5 :

Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Iba1 (ET1705-78, Green), anti-Olig2 (ET1604-29, White) and anti-TBR1 (ET1702-97, Red) on brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1705-78 (1/2,000 dilution), ET1604-29 (1/1,000 dilution) and ET1702-97 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_6.jpg

Pic legend6 :

Western blot analysis of Olig2 on different lysates with Rabbit anti-Olig2 antibody (ET1604-29) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 36 kDa Exposure time: 5 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-29) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-Olig2 antibody (ET1604-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Olig2 antibody (ET1604-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Olig2 antibody (ET1604-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Olig2 antibody (ET1604-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_11.jpg

Pic legend11 :

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/200

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_12.jpg

Pic legend12 :

Immunocytochemistry analysis of mouse glial cells labeling Olig2 with Rabbit anti-Olig2 antibody (ET1604-29) at 1/200 dilution. Cells were fixed with 4% PFA (15 min), permeabilized with 0.25% TritonX-100 for 15 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4℃ with Rabbit anti-Olig2 antibody (ET1604-29) at at 1/200 dilution. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1604-29_13.jpg

Pic legend13 :

Western blot analysis of Olig2 on different lysates with Rabbit anti-Olig2 antibody (ET1604-29) at 1/5,000 dilution. Lane 1: Mouse brain (P0) tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 36 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-29) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage