product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-ERG Antibody [SP06-04]
catalog :
ET1604-21TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SP06-04
reactivity :
human, mouse, rat
application :
western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
ET1604-21TR
Target name :
Anti-ERG Antibody [SP06-04]
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,ChIP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human ERG aa 430-479 / 479.
Uniprot id :
SwissProt: P11308 Human;SwissProt: P81270 Mouse;Unigene: 47987 Rat
Host :
Rabbit
Clone number :
SP06-04
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Jurkat cell lysates, CRC, PC-3M, human colon carcinoma tissue, mouse brain tissue, mouse heart tissue, human spleen tissue, human kidney tissue.
Molecular wt :
54 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:100-1:500
Pic img4 :
ET1604-21_2.jpg
Pic img5 :
ICC staining of ERG in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1604-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Pic legend5 :
ET1604-21_3.jpg
Pic legend6 :
ICC staining of ERG in PC-3M cells (green).
Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1604-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Pic img7 :
ET1604-21_4.jpg
Pic img8 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend8 :
ET1604-21_5.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
ET1604-21_6.jpg
Pic img11 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend11 :
ET1604-21_7.jpg
Pic legend12 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img13 :
ET1604-21_8.jpg
Pic img14 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend14 :
ET1604-21_9.jpg
Pic legend15 :
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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