ERG | HUABIO ET1604-21-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ERG

catalog :

ET1604-21-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP06-04

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ERG on Jurkat cell lysates with Rabbit anti-ERG antibody (ET1604-21) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ERG on different lysates with Rabbit anti-ERG antibody (ET1604-21) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1604-21-50UL

Target name :

ERG

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ERG aa 430-479 / 479.

Uniprot id :

P11308>SwissProt: P11308 Human;SwissProt: P81270 Mouse;Unigene: 47987 Rat

Host :

Rabbit

Clone number :

SP06-04

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysates, CRC, PC-3M, human colon carcinoma tissue, mouse brain tissue, mouse heart tissue, human spleen tissue, human kidney tissue.

Molecular wt :

54 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Tissue: 1:200-1:500 ;IHC-P: 1:200-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_7.jpg

Pic legend7 :

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/200 dilution. The section was incubated with ET1604-21 for 32 mins at room temperature. The immunostaining was performed on a Roche Benchmark XT instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/ET1604-21_14.jpg

Pic legend14 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERG antibody (ET1604-21) at 1/200 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ET1604-21 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage