Phospho-STAT3 (Y705) | HUABIO ET1603-40 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-STAT3 (Y705)

catalog :

ET1603-40

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SZ43-01

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-STAT3 (Y705) on different lysates with Rabbit anti-Phospho-STAT3 (Y705) antibody (ET1603-40) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate Lane 5: A431 cell lysate Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 35 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-40) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Phospho-STAT3 (Y705) antibody (ET1603-40) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-40) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of Phospho-STAT3 (Y705) on different lysates with Rabbit anti-Phospho-STAT3 (Y705) antibody (ET1603-40) at 1/1,000 dilution. Lane 1: M‑NFS‑60 cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: L6 cell lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1603-40

Target name :

Phospho-STAT3 (Y705)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr705 of human STAT3.

Uniprot id :

P40763>SwissProt: P40763 Human;SwissProt: P42227 Mouse;SwissProt: P52631 Rat

Host :

Rabbit

Clone number :

SZ43-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate, Jurkat treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate, A431 treated with 100ng/mL EGF for 30 minutes cell lysate, human lung tissue, M‑NFS‑60 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate, Mouse heart tissue lysate, SK-Br-3 cell lysates, human kidney tissue.

Molecular wt :

Predicted band size: 88 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:500 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1603-40_4.jpg

Pic legend4 :

Western blot analysis of Phospho-STAT3 (Y705) on SK-Br-3 cell lysates with Rabbit anti-Phospho-STAT3 (Y705) antibody (ET1603-40) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-40) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1603-40_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phospho-STAT3 (Y705) antibody (ET1603-40) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-40) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1603-40_6.jpg

Pic legend6 :

Phospho-STAT3 (Y705) was immunoprecipitated from 0.2 mg HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate with ET1603-40 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1603-40 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate (input) Lane 2: Rabbit IgG instead of ET1603-40 in HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate Lane 3: ET1603-40 IP in HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1802

more info or order :

HUABIO product webpage