antibody

Anti-EGFR Antibody [SZ40-19]

ET1603-37TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SZ40-19

human, mouse, rat

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Anti-EGFR Antibody [SZ40-19]

Human,Mouse,Rat

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Non-conjugated

Recombinant protein within human EGFR aa 1175-1210/1210.

SwissProt: P00533 Human;SwissProt: Q01279 Mouse;Unigene: 37227 Rat

Rabbit

SZ40-19

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HeLa cell lysate, A431 cell lysate, MDA-MB-468 cell lysate, A431, Hela, HepG2, human tonsil tissue, human kidney tissue, mouse liver tissue, mouse skin tissue, mouse kidney tissue.

Predicted 134 kDa, observed 150 kDa.

Secreted and Cell membrane.

1 mg/mL.

WB: 1:2,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: 1:10-1:50 ;FC: 1:1,000

ET1603-37_2.jpg

Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (ET1603-37) at 1/500 dilution and competitor's antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (ET1603-37) at 1/500 dilution and competitor's antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

ET1603-37_3.jpg

Flow cytometric analysis of A431 cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1603-37, red) at 1/1,000 dilution and competitor's antibody (red) at 1/200 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

ET1603-37_4.jpg

ICC staining of EGFR in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1603-37_5.jpg

ICC staining of EGFR in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1603-37_6.jpg

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-37_7.jpg

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-37_8.jpg

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-37_9.jpg

Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-37_10.jpg

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.