antibody

Anti-Bax Antibody [SZ3-07]

ET1603-34TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SZ3-07

human, mouse, rat

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Anti-Bax Antibody [SZ3-07]

Human,Mouse,Rat,Goat

WB,IHC-P,IP,FC

Non-conjugated

Synthetic peptide within Human Bax aa 1-50 / 192.

SwissProt: Q07812 Human;SwissProt: Q07813 Mouse;SwissProt: Q63690 Rat

Rabbit

SZ3-07

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, bEnd.3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, human tonsil tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse prostate tissue, mouse kidney tissue, human prostate tissue, HeLa.

Predicted band size: 21 kDa

Mitochondrion membrane, Cytoplasm.

1 mg/mL.

WB: 1:5,000-1:20,000 ;IHC-P: 1:50-1:200 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

ET1603-34_2.jpg

All lanes: Western blot analysis of Bax with anti-Bax antibody [SZ3-07] (ET1603-34) at 1/1,000 dilution. Lane 1/2: Wild-type Hela whole cell lysate (20 µg). Lane 3/4: Bax knockout Hela whole cell lysate (20 µg). ET1603-34 was shown to specifically react with Bax in wild-type Hela cells. No band was observed when Bax knockout samples were tested. Wild-type and Bax knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1603-34, 1/1,000) and Loading control antibody (Rabbit anti-β-actin, R1207-1, 1/1,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

ET1603-34_3.jpg

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bax antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-34_4.jpg

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Bax antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-34_5.jpg

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bax antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-34_6.jpg

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Bax antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-34_7.jpg

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Bax antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-34_8.jpg

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Bax antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1603-34_9.jpg

Flow cytometric analysis of HeLa cells labeling Bax. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1603-34, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).