Phospho-c-Myc (T58 + S62) | HUABIO ET1603-24 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-c-Myc (T58 + S62)

catalog :

ET1603-24

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SZ02-06

reactivity :

human, mouse

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-c-Myc (T58 + S62) on different lysates with Rabbit anti-Phospho-c-Myc (T58 + S62) antibody (ET1603-24) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 treated with 25μM MG-132 for 4 hours cell lysate Lane 3: HCT 116 treated with 25μM MG-132 for 4 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-24) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-c-Myc (T58 + S62) on different lysates with Rabbit anti-Phospho-c-Myc (T58 + S62) antibody (ET1603-24) at 1/1,000 dilution. Lane 1: EL4 cell lysate Lane 2: EL4 treated with 25μM MG-132 for 4 hours cell lysate Lane 3: EL4 treated with 25μM MG-132 for 4 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 1 minute 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of Phospho-c-Myc (T58 + S62) was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1603-24, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

ET1603-24

Target name :

Phospho-c-Myc (T58 + S62)

Species reactivity :

Human,Mouse

Applications :

WB,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of Human c-Myc.

Uniprot id :

P01106>SwissProt: P01106 Human;SwissProt: P01108 Mouse

Host :

Rabbit

Clone number :

SZ02-06

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT 116 cell lysate, HCT 116 treated with 25μM MG-132 for 4 hours cell lysate, EL4 cell lysate, EL4 treated with 25μM MG-132 for 4 hours cell lysate, K562.

Molecular wt :

Predicted band size: 49 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage