ERK2 | HUABIO ET1603-23 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ERK2

catalog :

ET1603-23

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SZ25-01

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ERK2 on different lysates with Rabbit anti-ERK2 antibody (ET1603-23) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ERK2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-23) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ERK2 on different lysates with Rabbit anti-ERK2 antibody (ET1603-23) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-23) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining of ERK2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1603-23

Target name :

ERK2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ERK2 aa 311-360 / 360.

Uniprot id :

P28482>SwissProt: P28482 Human;SwissProt: P63085 Mouse;SwissProt: P63086 Rat

Host :

Rabbit

Clone number :

SZ25-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, Hela, MCF-7, NIH/3T3, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cytoplasm, Nucleus, Cytoskeleton, Membrane, Cell junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-Fr: 1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_4.jpg

Pic legend4 :

ICC staining of ERK2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_5.jpg

Pic legend5 :

ICC staining of ERK2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ERK2 antibody (ET1603-23) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-23) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-ERK2 antibody (ET1603-23) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-23) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-ERK2 antibody (ET1603-23) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-23) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_9.jpg

Pic legend9 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling ERK2 with Rabbit anti-ERK2 antibody (ET1603-23). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1603-23, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_10.jpg

Pic legend10 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling ERK2 with Rabbit anti-ERK2 antibody (ET1603-23). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1603-23, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_11.jpg

Pic legend11 :

Flow cytometric analysis of NIH/3T3 cells labeling ERK2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1603-23, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_12.jpg

Pic legend12 :

Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling ERK2 with Rabbit anti-ERK2 antibody (ET1603-23) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-23, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1603-23_13.jpg

Pic legend13 :

Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling ERK2 with Rabbit anti-ERK2 antibody (ET1603-23) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-23, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage