Tau | HUABIO ET1603-2 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Tau

catalog :

ET1603-2

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SZ03-03

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Tau with Rabbit anti-Tau antibody (ET1603-2). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-2, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

image 2 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (ET1603-2). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-2, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

image 3 :

Western blot analysis of Tau on mouse brain tissue lysates with Rabbit anti-Tau antibody (ET1603-2) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 50 kDa Exposure time: 3 minutes 49 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1603-2

Target name :

Tau

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Tau aa 680-730.

Uniprot id :

P10636>SwissProt: P10636 Human;SwissProt: P10637 Mouse;SwissProt: P19332 Rat

Host :

Rabbit

Clone number :

SZ03-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse cerebral cortex tissue, mouse hippocampus tissue, mouse brain tissue lysates, rat brain tissue lysates, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Nucleus, Cytoplasm, Cell membrane, Cell projection, Cytoskeleton, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200 ;IHC-Fr: 1:50 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1603-2_4.jpg

Pic legend4 :

Western blot analysis of Tau on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1603-2_5.jpg

Pic legend5 :

Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (ET1603-2) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Tau KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-2) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1603-2_6.jpg

Pic legend6 :

Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with ET1603-2 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1603-2 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: ET1603-2 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of ET1603-2 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1603-2_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (ET1603-2) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-2) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1603-2_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tau antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage