GFP | HUABIO ET1602-7 product information

product summary

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company name :

HUABIO

product type :

antibody

product name :

GFP

catalog :

ET1602-7

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR48-02

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GFP on recombinant GFP protein using anti-GFP antibody at 1/5,000 dilution.

image 2 :

GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1602-7 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: Rabbit IgG instead of ET1602-7 in GFP Tag fusion protein lysate. Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate. Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate. Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate. Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate. Lane 7: R1312-2 IP in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1602-7) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1602-7

Target name :

GFP

Species reactivity :

Species independent

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.

Uniprot id :

P42212>SwissProt: P42212 Aequorea victoria

Host :

Rabbit

Clone number :

SR48-02

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant GFP protein, GFP Tag fusion protein lysate.

Molecular wt :

Predicted band size: 27 kDa

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:10,000 ;IHC-P:1:2,000-1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IP: 2-5 µg/ml

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1602-7_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1602-7) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-7) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage