Anti-Active Caspase-3 Antibody [SR01-02] | HUABIO ET1602-47TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Active Caspase-3 Antibody [SR01-02]

catalog :

ET1602-47TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR01-02

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1602-47TR

Target name :

Anti-Active Caspase-3 Antibody [SR01-02]

Species reactivity :

Human,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Caspase-3 aa 28-67 / 277.

Uniprot id :

SwissProt: P42574 Human

Host :

Rabbit

Clone number :

SR01-02

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Camptothecin (2 μM) treated Jurkat cell lysate, Hela, PC-3M, human colon carcinoma tissue, human placenta tissue.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Pic img4 :

ET1602-47_2.jpg

Pic img5 :

Western blot analysis of Active Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-47, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Camptothecin (2 μM) treated Jurkat cell lysate Lane 2: Untreated Jurkat cell lysate

Pic legend5 :

ET1602-47_3.jpg

Pic legend6 :

ICC staining of Active Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

ET1602-47_4.jpg

Pic img8 :

ICC staining of Active Caspase-3 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend8 :

ET1602-47_5.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

ET1602-47_6.jpg

Pic img11 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Active Caspase-3 antibody (ET1602-47) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-47) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage