Anti-MMP12 Antibody [SR03-23] | HUABIO ET1602-42TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-MMP12 Antibody [SR03-23]

catalog :

ET1602-42TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR03-23

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1602-42TR

Target name :

Anti-MMP12 Antibody [SR03-23]

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human MMP12 aa 421-470 / 470.

Uniprot id :

SwissProt: P39900 Human;SwissProt: P34960 Mouse;SwissProt: Q63341 Rat

Host :

Rabbit

Clone number :

SR03-23

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, Hela cell lysate, MCF-7 cell lysate, THP-1 cell lysate, human breast carcinoma tissue lysate, mouse lung tissue lysate, rat lung tissue lysate.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:20-1:50 ;IF-Tissue: 1:20-1:50 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Pic img4 :

ET1602-42_2.jpg

Pic img5 :

Western blot analysis of MMP12 on different lysates with Rabbit anti-MMP12 antibody (ET1602-42) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si MMP12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. ET1602-42 was shown to specifically react with MMP12 in Hela-si NT cells. Weakened band was observed when Hela-si MMP12 sample was tested. Hela-si NT and Hela-si MMP12 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-42, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Pic legend5 :

ET1602-42_3.jpg

Pic legend6 :

Western blot analysis of MMP12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: MCF-7 cell lysate Lane 4: THP-1 cell lysate

Pic img7 :

ET1602-42_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic legend8 :

ET1602-42_5.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-42, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

ET1602-42_6.jpg

Pic img11 :

Flow cytometric analysis of MMP12 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-42, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

more info or order :

HUABIO product webpage