antibody

Anti-Caspase-3 Antibody [SR03-01]

ET1602-39TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SR03-01

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Anti-Caspase-3 Antibody [SR03-01]

Human

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Non-conjugated

Synthetic peptide within human Caspase-3 aa 60-100.

SwissProt: P42574 Human

Rabbit

SR03-01

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, Hela, human tonsil tissue, human spleen tissue.

Predicted band size: 32 kDa

Cytoplasm

1 mg/mL.

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50 ;FC: 1:20-1:50 ;IP: Use at an assay dependent concentration.

ET1602-39_2.jpg

All lanes: Western blot analysis of Caspase-3 with anti-Caspase-3 antibody [SR03-01] (ET1602-39) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (20 µg). Lane 2: Caspase-3 knockout Hela whole cell lysate (20 µg). ET1602-39 was shown to specifically react with Caspase-3 in wild-type Hela cells. No band was observed when Caspase-3 knockout sample was tested. Wild-type and Caspase-3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-39, 1/500) and Loading control antibody (Rabbit anti-β-actin, R1207-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

ET1602-39_3.jpg

ICC staining of Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1602-39_4.jpg

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1602-39_5.jpg

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.