p53 (acetyl K370) | HUABIO ET1602-38 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p53 (acetyl K370)

catalog :

ET1602-38

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR40-09

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p53 (acetyl K370) on different lysates with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 500ng/mL TSA for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 4 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of p53 (acetyl K370) on different lysates with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/1,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: C6 treated with 1μM TSA for 18 hours cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of MCF-7 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

ET1602-38

Target name :

p53 (acetyl K370)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human p53 aa 343-392 / 392 (acetyl K370).

Uniprot id :

P04637>SwissProt: P04637 Human;SwissProt: P02340 Mouse;SwissProt: P10361 Rat

Host :

Rabbit

Clone number :

SR40-09

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 treated with 500ng/mL TSA for 4 hours cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, MCF-7, PANC-1, HeLa cells treated with 500ng/mL TSA for 4 hours.

Molecular wt :

Predicted band size: 53 kDa

Subcellular location :

Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1602-38_4.jpg

Pic legend4 :

Immunocytochemistry analysis of PANC-1 cells labeling p53 (acetyl K370) with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 (acetyl K370) antibody (ET1602-38) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1602-38_5.jpg

Pic legend5 :

Flow cytometric analysis of HeLa cells treated with 500ng/mL TSA for 4 hours labeling p53 (acetyl K370). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-38, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage