Histone H2A (acetyl K9) | HUABIO ET1602-34 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H2A (acetyl K9)

catalog :

ET1602-34

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR4-15

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H2A(acetyl K9) on different cell lysates using anti-Histone H2A(acetyl K9) antibody at 1/500 dilution. Positive control: Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates Lane 2: Untreated HeLa whole cell lysates

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Histone H2A (acetyl K9) antibody (ET1602-34) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-34) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Histone H2A (acetyl K9) antibody (ET1602-34) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-34) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1602-34

Target name :

Histone H2A (acetyl K9)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,ChIP,CUT&Tag-seq

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Histone H2A aa aa 1-50 / 130 (acetyl K9) conjugated to Keyhole Limpet Haemocyanin (KLH).

Uniprot id :

P04908>SwissProt: P04908 Human;SwissProt:Q93077 Human;SwissProt: Q99878 Human;SwissProt: C0HKE6 Mouse;SwissProt: C0HKE1 Mouse;SwissProt: P02262 Rat

Host :

Rabbit

Clone number :

SR4-15

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates, A549, MCF-7, human tonsil tissue, human colon tissue, rat colon tissue.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:1,000-1:5,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1602-34_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H2A (acetyl K9) antibody (ET1602-34) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-34) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1602-34_5.jpg

Pic legend5 :

ICC staining of Histone H2A (acetyl K9) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1602-34_6.jpg

Pic legend6 :

ICC staining of Histone H2A (acetyl K9) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1602-34_7.jpg

Pic legend7 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2A (acetyl K9) (ET1602-34) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage