Anti-Phospho-Histone H2A.X(S139) Antibody [SR33-09] | HUABIO ET1602-2TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Phospho-Histone H2A.X(S139) Antibody [SR33-09]

catalog :

ET1602-2TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR33-09

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1602-2TR

Target name :

Anti-Phospho-Histone H2A.X(S139) Antibody [SR33-09]

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.

Uniprot id :

SwissProt: P16104 Human;SwissProt: P27661 Mouse;Unigene:2850 Rat

Host :

Rabbit

Clone number :

SR33-09

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, HeLa treated with UV for 2 hours whole cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours whole cell lysate, HepG2 cell lysate–treated with etoposide, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue, human colon carcinoma tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus, Chromosome

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:50-1:500

Pic img4 :

ET1602-2_2.jpg

Pic img5 :

Western blot analysis of Phospho-Histone H2A.X(S139) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate–treated with etoposide Lane 2: HepG2 cell lysate–untreated

Pic legend5 :

ET1602-2_3.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

ET1602-2_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic legend8 :

ET1602-2_5.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

ET1602-2_6.jpg

Pic img11 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic legend11 :

ET1602-2_7.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage