CCR7 | HUABIO ET1602-22-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CCR7

catalog :

ET1602-22-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR36-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of CCR7 with anti-CCR7 antibody (ET1602-22) at 1:5,000 dilution. Lane 1/2: Wild-type Hela whole cell lysate (10 µg). Lane 3/4: CCR7 fragment 1 knockdown Hela whole cell lysate (10 µg). Lane 5/6: CCR7 fragment 2 knockdown Hela whole cell lysate (10 µg). ET1602-22 was shown to specifically react with CCR7 in wild-type Hela cells. Weakened was observed when CCR7 knockdown sample was tested. Wild-type and CCR7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-22, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CCR7 on different lysates with Rabbit anti-CCR7 antibody (ET1602-22) at 1/5,000 dilution. Lane 1: HDLM-2 cell lysate Lane 2: Jurkat cell lysate Lane 3: Daudi cell lysate Lane 4: MCF7 cell lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse pancreas tissue lysate (low expression) Lane 7: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-22) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of CCR7 on different lysates with Rabbit anti-CCR7 antibody (ET1602-22) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lane 3: EL4 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lane 6: Rat spleen tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1602-22-50UL

Target name :

CCR7

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CCR7 aa 13-62 / 378.

Uniprot id :

P32248>SwissProt: P32248 Human;SwissProt: P47774 Mouse;Unigene:229736 Rat

Host :

Rabbit

Clone number :

SR36-04

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HDLM-2 cell lysate, Jurkat cell lysate, Daudi cell lysate, MCF7 cell lysate, mouse spleen tissue lysate, mouse pancreas tissue lysate, rat spleen tissue lysate, Raji cell lysate, EL4 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Daudi, RAW264.7, C6, human tonsil tissue, human spleen tissue.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:200-1:500 ;IF-Tissue: 1:200 ;IHC-P: 1:200 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD),Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1602-22_4.jpg

Pic legend4 :

Immunocytochemistry analysis of Daudi cells labeling CCR7 with Rabbit anti-CCR7 antibody (ET1602-22) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCR7 antibody (ET1602-22) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1602-22_5.jpg

Pic legend5 :

Immunocytochemistry analysis of RAW264.7 cells labeling CCR7 with Rabbit anti-CCR7 antibody (ET1602-22) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCR7 antibody (ET1602-22) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1602-22_6.jpg

Pic legend6 :

Immunocytochemistry analysis of C6 cells labeling CCR7 with Rabbit anti-CCR7 antibody (ET1602-22) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCR7 antibody (ET1602-22) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1602-22_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1602-22_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1602-22_9.jpg

Pic legend9 :

CCR7 was immunoprecipitated from 0.2 mg Daudi cell lysate with ET1602-22 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1602-22 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Daudi cell lysate (input) Lane 2: ET1602-22 IP in Daudi cell lysate Lane 3: Rabbit IgG instead of ET1602-22 in Daudi cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1802

more info or order :

HUABIO product webpage