CD9 | HUABIO ET1601-9-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD9

catalog :

ET1601-9-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA35-08

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HCT 116 cell lysate Lane 5: HepG2 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: A375 cell lysate Lane 8: B16-F1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of CD9 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of CD9 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1601-9-50UL

Target name :

CD9

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD9 aa 179-228 / 228.

Uniprot id :

P21926>SwissProt: P21926 Human;SwissProt: P40240 Mouse;SwissProt: P40241 Rat

Host :

Rabbit

Clone number :

SA35-08

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, HCT 116 cell lysate, HepG2 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, B16-F1 cell lysate, SW480, CRC, human tonsil tissue, human spleen tissue, human kidney tissue, mouse brain tissue, mouse spleen tissue.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cell membrane, Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50 ;IHC-P: 1:200-1:1,000 ;IP: 1-2μg/sample ;FC:1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1601-9_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-9, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1601-9_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-9, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1601-9_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD9 antibody (ET1601-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1601-9_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD9 antibody (ET1601-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1601-9_8.jpg

Pic legend8 :

Flow cytometric analysis of B16-F1 cells labeling CD9. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ET1601-9, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1601-9_9.jpg

Pic legend9 :

CD9 was immunoprecipitated from 0.2 mg K-562 cell lysate with ET1601-9 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-9 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input) Lane 2: ET1601-9 IP in K-562 cell lysate Lane 3: Rabbit IgG instead of ET1601-9 in K-562 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 11 seconds; ECL: K1801

more info or order :

HUABIO product webpage