Cytokeratin 20 | HUABIO ET1601-8-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 20

catalog :

ET1601-8-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA35-03

reactivity :

human, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cytokeratin 20 on different lysates with Rabbit anti-Cytokeratin 20 antibody (ET1601-8) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: LoVo cell lysate Lane 2: CRC cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 48/50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-8) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of Cytokeratin 20 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-8, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cytokeratin 20 antibody (ET1601-8) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-8) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1601-8-50UL

Target name :

Cytokeratin 20

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Cytokeratin 20 aa 375-424 / 424.

Uniprot id :

P35900>SwissProt: P35900 Human;SwissProt: P25030 Rat

Host :

Rabbit

Clone number :

SA35-03

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HT-29 cell lysate, LoVo cell lysate, CRC cell lysate, CRC, human colon carcinoma tissue, human small intestine tissue, rat small intestine tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/ml.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:500 ;FC: 1:50 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1601-8_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Cytokeratin 20 antibody (ET1601-8) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1601-8_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Cytokeratin 20 antibody (ET1601-8) at 1/200 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1601-8_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1601-8_7.jpg

Pic legend7 :

Flow cytometric analysis of Cytokeratin 20 was done on CRC cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1601-8, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1601-8_8.jpg

Pic legend8 :

Cytokeratin 20 was immunoprecipitated from 0.2 mg HT-29 cell lysate with ET1601-8 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-8 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: ET1601-8 IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of ET1601-8 in HT-29 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801

more info or order :

HUABIO product webpage