Anti-JNK1+JNK2+JNK3 Antibody [SA43-06] | HUABIO ET1601-28TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-JNK1+JNK2+JNK3 Antibody [SA43-06]

catalog :

ET1601-28TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA43-06

reactivity :

human, mouse, rat, bovine, zebrafish

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1601-28TR

Target name :

Anti-JNK1+JNK2+JNK3 Antibody [SA43-06]

Species reactivity :

Human,Mouse,Rat,Cow,Monkey,Zebrafish

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human JNK1 aa 1-423 / 427.

Uniprot id :

SwissProt: P45983 Human;SwissProt: P45984 Human;SwissProt: P53779 Human;SwissProt: Q61831 Mouse;SwissProt: Q91Y86 Mouse;SwissProt: Q9WTU6 Mouse;SwissProt: P49185 Rat;SwissProt: P49186 Rat;SwissProt: P49187 Rat

Host :

Rabbit

Clone number :

SA43-06

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, NIH/3T3, rat brain tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 48/53 kDa

Subcellular location :

Cytoplasm, Nucleus, Membrane, Mitochondrion

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:400 ;IP: Use at an assay dependent concentration.

Pic img4 :

ET1601-28_2.jpg

Pic img5 :

ICC staining of JNK1+JNK2+JNK3 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend5 :

ET1601-28_3.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

ET1601-28_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK1+JNK2+JNK3 antibody (ET1601-28) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-28) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage