Alas1 | HUABIO ET1601-26 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Alas1

catalog :

ET1601-26

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA70-04

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Alas1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 65 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: THP-1 cell lysate Lane 3: HeLa cell lysate Lane 4: JAR cell lysate Lane 5: Raji cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 65 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of Hela cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

ET1601-26

Target name :

Alas1

Species reactivity :

Human

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Alas1 aa 510-640 / 640.

Uniprot id :

P13196>SwissProt: P13196 Human

Host :

Rabbit

Clone number :

SA70-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, HepG2 cell lysate, THP-1 cell lysate, HeLa cell lysate, JAR cell lysate, Raji cell lysate, Hela, HepG2, MCF-7.

Molecular wt :

Predicted band size: 71 kDa

Subcellular location :

Mitochondrion matrix

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1601-26_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HepG2 cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1601-26_5.jpg

Pic legend5 :

Immunocytochemistry analysis of MCF-7 cells labeling Alas1 with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alas1 antibody (ET1601-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage