Ras | HUABIO ET1601-16 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ras

catalog :

ET1601-16

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA39-05

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (ET1601-16) at 1/1,000 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: Neuro-2a cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Ras with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET1601-16

Target name :

Ras

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Ras aa 7-56 / 189.

Uniprot id :

P01111>SwissProt: P01111 Human;SwissProt: P01112 Human;SwissProt: P01116 Human;SwissProt: P08556 Mouse;SwissProt: P32883 Mouse;SwissProt: Q61411 Mouse;SwissProt: P08644 Rat;SwissProt: P20171 Rat;SwissProt: Q04970 Rat

Host :

Rabbit

Clone number :

SA39-05

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, MCF7, NIH/3T3, PC-12.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Cytoplasm, Cell membrane, Golgi apparatus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1601-16_4.jpg

Pic legend4 :

Immunocytochemistry analysis of PC-12 cells labeling Ras with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1601-16_5.jpg

Pic legend5 :

Flow cytometric analysis of MCF7 cells labeling Ras. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-16, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1601-16_6.jpg

Pic legend6 :

Flow cytometric analysis of NIH/3T3 cells labeling Ras. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-16, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1601-16_7.jpg

Pic legend7 :

Flow cytometric analysis of PC-12 cells labeling Ras. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-16, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1601-16_8.jpg

Pic legend8 :

Ras was immunoprecipitated from 0.2 mg MCF7 cell lysate with ET1601-16 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-16 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: ET1601-16 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of ET1601-16 in MCF7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801

more info or order :

HUABIO product webpage